Technical Information Magazine 202304-02 Optical-PTIR
This paper presents examples of measuring non-fixed cells under physiological conditions to visualize the distribution and localization of cellular components (such as proteins and nucleic acids).
The technical information magazine The TRC News provides the latest information on analytical techniques that are useful for research and development, solving production troubles, and quality control. **Abstract** Infrared spectroscopy is a method for obtaining information about molecular structure by measuring infrared absorption due to molecular vibrations. Utilizing photothermal conversion, O-PTIR (Optical Photothermal IR Spectroscopy) enables infrared analysis with sub-micron spatial resolution and non-contact measurement. This paper presents a case study of measuring non-fixed cells under physiological conditions to visualize the distribution and localization of cellular components (such as proteins and nucleic acids). **Table of Contents** 1. Introduction 2. Overview and Features of O-PTIR 3. Analysis of Suspended Living Cells (Budding Yeast) in Water - Comparison of Intracellular Spectra - 4. Analysis of Suspended Living Cells (Budding Yeast) in Water - Distribution of Cellular Components - 5. Conclusion
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